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Finally, when cells were propagated in osteogenic medium and injected into the tibial muscles of SCID mice, no overtly mineralized bone tissue emerged.
The inoculum was adjusted to 5.0 log10 PFU (corresponding to 7.8 log10 RNA) in 1 ml of RPMI-1640 medium and injected intravenously (IV) to simulate direct IV mosquito feeding61 in the saphenous vein of four adult pregnant animals under aseptic conditions.
This procedure was repeated for three times to remove LB medium, and then the cell pellet was suspended with sterilized culture medium and injected into serum bottles with a fixed concentration.
All cells were grown in Roswell Park Memorial Institute (RPMI) medium supplemented with 10%% fetal bovine serum, 100 IU/mL penicillin, and 50 μg/mL streptomycin in a humidified incubator with 5%% CO2 at 37 °C, and then, 5 10 × 106 cells were resuspended in 200 μl RPMI medium and injected s.c. into the flank of nude mice.
Control cells and transformed cells were suspended in 5 µl of culture medium and injected into brains of 5∼8 week-old female nude mice that had been anesthetized with 10% pentobarbital.
Cells (ySL542) expressing Rod1-GFP were grown on lactate medium and injected into a microfluidics device in lactate medium.
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There was a significant difference between the survival of fish injected with PBS medium and fish injected with the Vibrio sp. isolate (Table 1).
For each porous medium, grout injection velocity and injected mass were measured.
Cells were then washed, placed in plain minimal essential medium, and then injected into human thymic implants in SCID-hu mice.
For each graft, approximately 106 iPS cells were manually harvested, washed and resuspended in a 1.5 ml tube containing 300 µl hESC medium and then injected subcutaneously into female SCID mice (Charles River Laboratories International, Inc., Wilmington, MA, USA, http://www.criver.com).
Lentivirus-transfected cells (1 × 10) were suspended in serum-free medium and then injected into the involucrum of the spleen.
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