Sentence examples for medium and infected from inspiring English sources

Five hours after transfection, cells were washed with fresh medium and infected with HD Ads at multiplicities of infection (MOIs) ranging from 100 to 1000 infectious viral particles per cell.

Six hours after infection, the culture medium was replaced with fresh medium and infected cells were allowed to recover for 48 hours.

Cells were then washed with medium and infected with WSN at an MOI of approximately 0.001.

The next day, cells were washed with normal medium and infected with adenoviruses expressing wild-type PTEN, dominant negative PTEN, or LacZ.

Cells were then washed three times with antibiotic-free medium and infected with 24 hr old H. pylori cultures (ratio 1 AGS cell/250 bacterial cells) for times appropriate to the experiment.

Cells were washed with complete RPMI medium and infected with LVS (MOI of 80 120) or stimulated in parallel with E. coli LPS (10 µg/ml; Sigma-Aldrich #L2360) in triplicate in 0.1 0.2 ml of antibiotic-free medium for 4 h at 37°C, 6% CO2.

After infection for 24 hours, the medium was replaced with fresh medium and the infected cells were selected with puromycin (InvivoGen).

Total RNA was extracted from U. maydis cell grown in YEPSL medium and from infected leaves by excising 2 3 cm segments from below the injection holes.

Non-adherent particles were washed off using 2% RPMI 1640 medium and the infected cells overlaid with 20 mL of 2% RPMI 1640 and incubated again until full cytopathic effect was observed in five to six days.

After 48 h incubation at 37°C and 5% CO2 in cycloheximide-free medium, infected and treated cells were recovered and used to determine the infectivity yield and the IC50 value.

LDH in medium from cultures containing uninfected and infected tissues was measured at 24 h by CytoTox-ONE™ kit (Promega, Madison, WI) according to the manufacturer's instructions.