Sentence examples for medium and further from inspiring English sources

Strains were grown in liquid LB medium with appropriate antibiotics at 37 °C overnight with shaking at 220 r.p.m., then 50 μl culture was inoculated into 5 ml fresh liquid LB medium and further incubated till the OD600 reached 1.2.

It consisted in the self-assembly of F127, Ni2+ salt and RF polymer in an acidic medium and further carbonization, where the Ni2+ was captured by the network of F127/RF and further reduced into metallic Ni nanoparticles.

An amphiphilic diperoxide, palmitoyl (3-carboxy propionyl) sebacoyl diperoxide (PSD), was synthesised by thesuccessive condensations of sebacoyl chloride with peroxypalmitic acid and monoperoxysuccinic acid in a pyridine medium and further characterised by i.r., 1H n.m.r., MS as well as elemental analysis.

A detailed structural identification of molecules needs a preliminary extraction in a suitable medium and further purification before characterization.

Pulsatile chonic treatment was carried out by incubating cultured cells with BMP7 for 15 h followed by replacing with fresh medium and further cultures of two days.

In addition, design of fermentation medium and further optimization of cultivation conditions using a well reasoned strategy ( Kennedy and Krouse 1999, Berlec et al. 2008 ) could contribute to multiple increases of cell densities and expression productivities.

An overnight culture was diluted 75 to 100 times in fresh LB-medium and further cultured for 4 to 8 hrs, depending on the clone, until OD600 0.5 0.7 with chloramphenicol and other antibiotics as appropriate.

Fig. 1 Time course of CDW and Surfactin concentrations of B. subtilis DSM 10T during shake flask cultivations in medium B and further optimized medium C. The achieved CDW [g/L] is shown in a, whereas resulting Surfactin concentrations [g/L] are illustrated in b.

After 3 h, the cells were washed with culture medium twice, and further incubated in drug-free medium for 21 h to measure apoptosis induction or 45 h to measure cell viability.

Single colonies were replica plated onto rich YEA medium plates and further incubated at 18 °C or 32 °C.

Thus, the study rigorously improved PYC production through medium optimization and further demonstrated its agricultural and therapeutic applications.