Sentence examples for medium and fluorescence from inspiring English sources

Before the third wash in PBS, sections were counterstained with the nuclear dye 4',6-diamidino-2-phenylindole (DAPI) for 5 min. Sections were then transferred onto glass slides and coverslipped with Moviol mounting medium and fluorescence was visualized using a Olympus SZ61 Stereo microscope (Olympus Corporation, Tokyo, Japan) or a laser scanning confocal microscope (Zeiss, Axiovert LSM 710).

Days after transfection, 10% v/v alamarBlue was added to culture medium and fluorescence was read in technical duplicate 4 h after addition using Xenius instrument (Safas).

The Apo-One Caspase 3/7 reagent was added in a 1 1 ratio with medium and fluorescence was measured using Novostar plate reader at 521 nm.

Cell death was simultaneously measured by adding ethidium homodimer-1 (EthD1; 0.3 μM; Molecular Probes) to the culture medium, and fluorescence was measured at Ex530 nm/Em645 nm.

P. luminescens TT01 transformants carrying plasmids pBR-Cherry or pBR-Cherry-rpsM, respectively, were grown in complex medium, and fluorescence was analyzed microscopically.

Briefly, TK-1 cells were suspended in culture medium and fluorescence labeled by incubating TK-1 cells at 2 × 106 cells/ml with 0.02 mg fluorescein diacetate (FDA) (Sigma) at 37°C for 30 min. The cells were then washed twice with ice-cold HBSS, spun at 250 g for 5 min to remove unincorporated fluorescence and suspended in HBSS.

The samples were mounted in fluorescent mounting medium, and the fluorescence was observed under a fluorescence microscope (Leica DMI6000 B, Wetzlar, Germany).

After 30 minutes, the cells were washed and centrifuged twice with 200 µl cold RPMI1640 culture medium, and cell fluorescence was measured at a wavelength of 490 nm (A490) on a SPECTRAmax® Microplate Spectrofluorometer (Molecular Devices).

After 30 minutes, the cells in plate were washed and centrifuged twice with 200 µl cold RPMI1640 culture medium, and cell fluorescence was measured at a wavelength of 490 nm (A490) on a SPECTRAmax® Microplate Spectrofluorometer (Molecular Devices).

Cell death was simultaneously measured by adding ethidium homodimer-1 (EthD1, 0.3 μM, Molecular Probes) to the culture medium, and the fluorescence was measured at Ex530 nm/Em645 nm.

Pressure was applied using a diamond anvil cell with methanol/ethanol as the hydrostatic medium and ruby fluorescence as the pressure calibrant.