Sentence examples for medium and fixed from inspiring English sources

Infected cells were washed free of medium and fixed for 10 min in paraformaldehyde (3.5%).

After incubation, cells were washed with CO2-independent medium and fixed with PFA.

For both experiments cells were washed afterwards with medium and fixed with 1% formaldehyde.

For confocal microscopy, cells grown overnight on 8-well chamber slides (Nalge Nunc, Rochester, NY) were treated with HAMLET (10% was labeled with Alexa-Fluor 568 (Molecular Probes, Eugene, OR)) in serum-free medium and fixed in 3.7% formaldehyde.

Cells were then washed 3 times with culture medium and fixed with 4% paraformaldehyde for 15 min. Bound laminin was detected by immunofluorescence staining with anti-laminin-1 antibody.

For immunocytochemistry, cells were grown on eight-well chamber slides (1×105 cells/well for LS174T and 2×104 cells/well for all others) at 37°C in conventional culture medium, and fixed in 2% paraformaldehyde for 10 min at room temperature.

Harvey (1981) proposed that by adding a large velocity cap layer to a given layered medium and fixing the bottom, the Green's function of full-wave could be approximated with superposition of normal modes of surface waves.

Optimizing the culture medium and fixing the ratio of defatted soybean meal to potassium nitrate at 2 1 gave a fourfold increase (6.0 g/L) in ARA production [ 7].

7dpf embryos were anesthetized with tricaine (10 µg/ml) in embryo medium [42] and fixed in 4% paraformaldehyde in phosphate-buffered saline (PBS; pH 7.2) for 2 hours.

Cells were grown to log phase in YPD medium, harvested and fixed in methanol for 5 min at room temperature.

The cover slips were rinsed with warm medium twice and fixed with 3.7% formaldehyde in 37°C for 15 minutes.