Sentence examples for medium and evaluated from inspiring English sources

IL1-α was assayed in each sample of culture medium and evaluated by enzyme-linked immunosorbent assay.

Therefore to determine whether HIF might regulate EGF-R mRNA synthesis, we added cobalt chloride, a chemical inducer of HIF, to the neurosphere culture medium and evaluated the levels of EGF-R mRNA transcripts.

Cells were incubated for 30 min on ice with PE-conjugated murine anti-CD70 antibodies (BD Pharmingen, San Diego, CA, USA), washed with cold staining medium, and evaluated with a Becton Dickinson FACScan flow cytometer.

After incubation at 37 °C in 5 % CO2 for 2 h, cultures were supplemented with 1 ml IMDM plus FBS 20%% and BoosterExpress™ reagent (Gene Therapy Systems, San Diego, CA, USA) (final dilution 1 200) and incubated for 18 h, extensively washed in serum-containing medium, and evaluated for transduction efficiency by flow cytometry.

Then they were dried, covered by DePex mounting medium (Serva) and evaluated at magnification 400x using a BX60 microscope (Olympus).

Initial fermentation medium (FM1), single-factor optimum medium (FM2), and BBD optimum medium (FM3) were investigated and evaluated to validate the predicted optimal conditions (Table 4).

Murine 3T3 fibroblasts carrying the IGF-1 receptor were maintained in DMEM medium and were evaluated in different concentrations of IGF-1 by the CyQUANT NF Cell proliferation assay (Invitrogen Inc., San Diego, CA) and by hemocytometer counting methods.

In this study, we determined the optimal medium for sperm preservation and evaluated the effect of reduced glutathione in the fertilization medium on sperm fertility after cold storage.

For this reason, and also for not facing with the problem of degree of freedom, the medium units will be analyzed and evaluated alongside with large units.

However, in the approach described by Kroner the terms in the series were established by selecting a reference medium and numerically evaluating a complex series of nested convolution integrals.

Self-help treatments for OCD have been developed and evaluated previously [ 11- 14] with medium to large effect sizes.