Sentence examples for medium and estimated from inspiring English sources
The recombinant CSLP and CSLP-CBD were secreted into culture medium and estimated by SDS PAGE to be 22 and 27 kDa, respectively.
The aim of this work was to develop a practical method for determining diffusion coefficients in anisotropic medium and to estimate the overall diffusion coefficients of fluorescently labeled ATP in rat cardiomyocytes.
In this study we developed strain-specific molecular markers based on polymerase chain reaction amplification of sequence-characterized amplified regions (SCAR) in combination with dilution plating on semi-selective medium to detect and estimate density of propagules of a commercial strain of B. bassiana (strain GHA) in field samples.
The contents of cellulose, hemicelluloses and lignin in ramie stalk medium were estimated and the changes occurring during fructification were calculated.
Concentration of cells in the medium was estimated and cells were plated in a volume of 250 µl in 4-well plates (176740, Nunc, Thermo Scientific) or 35 µl in μ-clear 96-well plates (Greiner) coated with poly-D-lysine (Sigma Aldrich) at a concentration of 2×10 cells per ml.
Calculation of effects for 100% equipment rate The effect estimates for safety, mobility and comfort were used to calculate the overall low, medium and high estimates of the effect of the system.
Calculation of effects The effect estimates per mechanism (from steps 3 and 4) were used to calculate the overall low, medium and high estimate on the effect of the system.
Biomass in original medium and glutamic acid-containing medium was estimated by determining the dry weight of the mycelia.
Biofilms were generated from strains MG1655 and PHL628 (a) or MC4100 and PHL644 (b) with and without pSTB7 using the spin-down method, matured for 7 days in M63 medium and biomass was estimated using crystal violet staining.
Since these estimates are not precise, we developed a range for the multiplier to produce low, medium and high estimates.
The SCFA profile of the incubation medium was estimated after 72 h, and from these data the amount of ATP produced in vitro was predicted.
