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For swab samples, dacron-tipped applicators were inserted into the oropharyngeal cavity (behind the pouch) or into the cloaca and then submerged and swirled in vials containing 1 mL BA-1 medium and discarded.
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At selected time points the culture medium was aspirated and discarded.
Cells (1 × 10 per dish) were seeded on 10-cm dishes and allowed to attach overnight and then the medium was discarded and replenished with medium containing the DPD for incubation at 37°C for 3 days.
Cells (1 × 10 per dish) were seeded on 10-cm dishes and allowed to attach overnight, and then the medium was discarded and replenished with medium containing DPD for incubation at 37°C for 3 days.
Every 3 4 days, half of the medium was discarded and replenished by fresh medium and cytokines.
After 24 hour serum starvation, medium were discarded and AoSMCs washed and resuspended with fresh DMEM medium.
After cell seeding and treatment (0 – 5 mg/ml), the medium was discarded and replaced with equal amount of neutral red medium to each well of the plate.
Break all eight of the eggs into a medium-sized bowl and discard the shells.
The medium was discarded and replaced with DOX solution and DOX nanoparticles.
First, the culture medium was discarded and the cells were trypsinized and washed with PBS.
Next, the culture medium was discarded, and the cells were trypsinized, centrifuged, and washed three times with PBS buffer.
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