Sentence examples for medium and differentiated from inspiring English sources

The wild type mESCs and Clock knockout mESCs were cultured in the mESC maintenance medium and differentiated spontaneously for 15 days in the differentiation medium.

10 Briefly, cells were cultured in RPMI 1640 culture medium and differentiated.

Cells were maintained overnight with this medium and differentiated into macrophages with hM-CSF (20 ng/ml; PeproTech) for 7 days.

To assess whether the binding of Cbx7 to H3K27me3 was specific for ESCs, we performed H3K27me3 pulldown (PD) assays using nuclear extracts from mouse ESCs grown in "heavy" medium and differentiated ESCs or mouse embryo fibroblasts (MEFs), grown in "light" medium.

NKX2-5 eGFP/w hiPSCs (R.P.D. and C.L.M., unpublished data) were maintained in Essential 8 medium (Life Technologies) and differentiated as previously described (van den Berg et al., 2015).

Following the PMA treatment, the medium was replaced with fresh medium, and the differentiated cells were incubated for an additional 24 h prior to use.

> According to the previous studies of histological differentiation and malignant degree of gastric carcinoma, non-mucinous adenocarcinoma (papillary adenocarcinoma and high, medium and low differentiated tubular adenocarcinoma) were assigned as 1 3 points, respectively.

Single cells that had previously been cultured in serum/LIF, 2i, or 2i/LIF were sorted into medium without LIF and differentiated under clonal conditions.

We therefore tested if cells could be grown in a completely humanized base medium with hKSR and the growth factors (Invitrogen) present in the defined medium, and could be differentiated appropriately.

To prevent spontaneous differentiation, the iPS culture medium was replaced daily and differentiated cells in cultures manually removed.

The medium was changed daily, and differentiated cells were moved manually after 7 days.