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Significantly, three tendencies for the folded/unfolded equilibrium are observed: increasing solubility and polarity of the medium and decreasing temperature results in a higher extent of folding.
In the present study, we followed a different approach consisting in optimization of a method for DyP expression as active soluble forms by adding hemin to the E. coli culture medium and decreasing the growth temperature to 16 °C after IPTG induction.
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In our study, HNE triggered considerable cell death in stressed ARPE-19 cells as observed by the increased LDH levels in the medium, and decreased metabolic activity measured by the MTT test.
The water absorbency of SAP was strongly affected by the salinity of the medium and decreased as the salinity increased.
Xie et al. [52] showed that PEG coating on Fe3O4 NPs protect the NPs from aggregation in cell culture medium and decreased nonspecific uptake by macrophages.
CmADH2A deficiency slightly increased CmADH1 expression in glucose medium and decreased CmADH1 expression in other substrates.
nerii caused necrotic lesions when infiltrated into tobacco leaves, induced H2O2 release from tobacco cells in culture medium, and decreased cytosolic ascorbate peroxidase (APX), one of the main enzymes for ROS scavenging in plant cells; EPS extracts from the related plant pathogen P. caryophylli had no such effects [38].
Moreover, metabolically generated bicarbonate ions accumulate in the medium and decrease the pH-value [ 15].
Aggregation occurs in the presence of an elevated concentration of macromolecules in the suspending medium and decreased blood flow [ 1].
In comparison with untreated control cells, the cell cultures pretreated with HSME displayed larger amounts of formazan after the MTT viability test and lower levels of LDH in the culture medium and decreased amount of intracellular ROS.
A general tendency of increased expression from low to medium U, and decreased expression from medium to high U concentration was found for most of the target genes tested.
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CEO of Professional Science Editing for Scientists @ prosciediting.com