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The rates of ammonia elimination were established by calculating the changes in absolute molecular amounts of ammonia in the medium and corrected for time and cell number.
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In the literature, blanks are computed on the basis of i) non-stained samples in order to estimate the autofluorescence background of the medium and correct for cellular autofluorescence [ 17, 49, 52, 62] or ii) the sum of the cell autofluorescence and the dye fluorescence in the medium [ 18, 24, 89].
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Fluorescence of the subsequently removed medium was measured using a Fluroskan Ascent plate reader (Labsystems, Helsinki, Finland) and corrected for background.
A displacement seismogram was corrected for medium attenuation using the following equation:, where u and u c are the observed and corrected displacements, respectively; ω is angular frequency; and l is the hypocentral distance.
Noted and corrected.
[21] and corrected manually.
Transformants were grown on solid selective medium, and the correct integration of the tagging construct was checked in positive candidates by colony PCR.
The determined aromatase activity was subtracted from background level (data from wells with medium only), corrected to cell protein concentration, and related to the solvent control (0.1% DMSO).
The resulting amount of HA in the culture medium was corrected for sample weight.
The lower concentration of potassium because of the absence of KH2PO4 in the phosphate-free medium was corrected by the addition of KCl.
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