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The production medium was different from M9 minimal medium, and contained yeast extract (Materials and methods).
The medium for batch and continuous cultivation was Minimal Medium and contained per liter: 4.5 g NH4Cl, 1.5 g KH2PO4, 0.5 KCl, 0.5 MgSO4·7H2O and 1 ml trace metal solution.
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The E11 deposits are of medium thickness and contain fine grains (coarse to medium sand).
"Metrio" or "metrios" is medium strength and contains up to 1 tsp.
Enhanced enzyme activity and gene expression were observed in both glucose-containing medium and medium containing only cellulose.
Cytokine concentrations did not differ between the two media containing BSA or between albumin-free medium and medium containing HSA.
Serum-free medium and medium containing 10% FBS, were supplemented with M-CSF and RANKL, and used as negative and positive controls, respectively.
All cytokine stimulations were performed in complete culture medium, defined as medium containing FBS and antibiotics.
Embryos were mounted in 1.5% low melting agarose and medium containing PTU and MESAB was added.
The agar plate contained BHI medium and a paper disc contained 20 μL of each stressor.
Solution 2 (medium stringency) contained 0.1× SSC and 0.1% SDS.
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