The follicular fluid was obtained from the largest follicle (> 18 mm) visualized on ultrasound before using any flushing medium and only consisted of fluid from one follicle.
They were more so in medium to large gaps and consisted of shade-intolerant and early successional species.
Preantral follicle isolation and washing medium consisted of Minimum Essential Medium (MEM) supplemented with steer serum (10%), glutamine (2 mM), sodium pyruvate (0.23 mM), hypoxanthine (2 mM) and gentamycin (50 μg/ml), respectively.
The dissection and culture medium consisted of 50% minimum essential medium eagle, 25% heat inactivated horse serum, 25% Hank's Balanced Salt Solution, supplemented with 100 units penicillin, 100 μg streptomycin, 2 mM glutamine, 5 mg/ml glucose, and 25 mM 4- 2-hydroxyethyl -1-piperazineethanesulfonic acid (HEPES).
The low group consisted of values < LOD for each metabolite, whereas the medium and high groups consisted of equal-sized bins among the detected values.
The control medium (CM) was RPMI 1640 and 10% FBS, and the survival medium (SM) consisted of CM supplemented with 500 pM granulocyte-macrophage colony-stimulating factor (GM-CSF), 10 ng/ml interleukin (IL -4, and 10 ng/ml TNF-α.
Cybrid cell lines and NT2 cell-line growth medium consisted of OPTIMEM medium supplemented with 10% heat-inactivated FBS, 10 000 U/ml penicillin and 10 µg/ml streptomycin.
Eruptive products are medium-K andesite to dacite and consist of the Older and Younger Kitadake and Older and Younger Minamidake stages.
The culture medium consisted of DMEM and Ham's medium (2∶1) supplemented with 10% fetal calf serum, 2 mM glutamine, 0.001% insulin, 5 mM HEPES, 0.3% glucose, and 1% antibiotic-antimycotic solution.
(Selection medium consisted of regeneration medium, pH 6.5, supplemented with 40 µg mL−1 hygromycin).
