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A feeder layer comprised of IMDM growth medium and compound was poured on top of the hardened cell mixture.
Two-week-old A. thaliana Col-0 seedlings (600 mg) were transferred to liquid ½ MS medium and compound 15 was added to a final concentration of 50 μM.
4 × 10 cells per well (in a 12-well plate) were resuspended in a 42 °C mixture of 0.35% ultrapure agarose (Life Technologies, 12200036), IMDM growth medium, and compound.
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Embryo medium and compounds were replenished daily.
Medium and compounds were refreshed every 2 3 days.
Fresh medium and compounds were added every 48 h, and cells were then trypsinized and counted after 48 and 96 h using a Coulter Z1 cell counter.
There are 377 compounds categorised as "low", 2029 compounds as "medium" and 1390 compounds as "high".
The tyre manufacturer brought its medium and supersoft compound tyres to the race, and the latter were marginal given the stresses involved, particularly on the right front tyre.
In the fluidized bed, the mass transfer coefficient (β, m/s) is calculated per unit of phase contact surface and determined by physical and process parameters of gaseous medium and solid compound.
The loaded disc was placed on the surface of medium and the compound was allowed to diffuse for 5 minutes and the plates were kept for incubation at 37°C for 24 hrs.
In our assay, a small aliquot of the pex5 Δ culture grown in a nutrient-rich medium containing 0.5% glucose and recovered from mid-logarithmic phase was transferred into each well of a 96-well master microplate containing the same growth medium and a compound from a commercially available library.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com