Surface-sterilized wild-type and mutant seeds were imbibed on MS/G medium and cold-treated at 4°C in dark for 2 days.
However, for various cell types, UW did not provide better protection than cell culture medium, and cold-induced cell injury in UW could also be greatly reduced by the addition of iron chelators [ 25, 32, 59].
IADT and AAPD guidelines for the management of dental trauma state that the physiological transportation media for avulsed teeth include Hank's Balanced Salt Solution (tissue culture medium), saline, and cold milk [ 9, 10, 19- 22].
The two mutants showed impaired cell growth on benomyl-containing medium and at cold temperatures, altered microtubule (MT) dynamics, and altered nucleus positioning and segregation.
Cells to be sorted were resuspended in complete medium and kept cold until sorting.
SKOV3.ip cells at log phase (2 or 5 × 10) were suspended in 0.2 ml of either physiological solution or a mixture (1 : 1) of growth medium and ice-cold Matrigel (BD Biosciences), and injected into the dorsum of female, 6- to 7-week-old SCID mice (Canese et al, 2012).
After labeling, the cells were washed twice in cold culture medium, and once with cold PBS.
Chilled PureCol Bovine Collagen Product 3 mg ml−1 (Nutacon BV, Leimuiden, The Netherlands) was mixed with 10-fold concentrated Dulbecco's minimal essential medium (Sigma-Aldrich) and cold 0.1 M sodium hydroxide (Sigma-Aldrich) at a ratio of 8 : 1 : 1.
The dissected tissue was collected in cold Neurobasal™ medium and rinsed twice with cold PBS.
Ground pork and miso udon ($9 for medium) is cold, sweet and meaty, a combination that isn't for everybody.
