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The flies were raised at 23 °C, 60%% humidity, and 12-h light/dark cycle on standard cornmeal-yeast medium and changed every 3 5 days.
Media was switched to fibroblast culture medium and changed every other day.
Following seeding, medium was replaced at day 2 by fresh growth medium and changed every 2 days over 9 14 days.
Cells were washed once with PBS after overnight culture in serum-containing medium and changed to serum-free and antibiotic-free medium immediately before infection.
After complete of the selection, medium in flasks containing attached transduced cells was replaced with fresh complete growth medium and changed every 3 ~ 4 days thereafter.
After incubation for two more days, the medium was replaced with the derivation medium and changed every 3 4 days thereafter.
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Studies showed that alcohols can affect the dielectric constant of the medium and change the crystal growth rate [21].
After irradiation, the medium was removed and changed to standard culture medium.
Thereafter 3FC medium was used and changed every 96 h.
Change the medium and you change the organisms.
Culture medium was then added and changed every 48 hours.
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