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Top chambers (culture inserts) were filled with serum-free medium, and bottom chambers were filled with medium containing 20% FBS as a chemoattractant.
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Samples for microbial characterization were collected from the filter medium (top and bottom layers) of the wetland, water influent and effluent at the end of Periods I (Jun Oct) and II (Nov Jan).
The top chambers of the trans-wells were filled with 0.2 ml of cells (5 × 105 cells/ml) in serum-free medium, and the bottom chambers were filled with 0.25 ml of RPMI 1640 medium containing 10 % FBS.
The values of the absorption coefficient (μa) of the homogeneous medium and the bottom part of the two-layer medium were varied from 0.029 to 0.085 cm−1 in steps of 0.014 cm−1, while their reduced scattering coefficient (μs') and both optical properties of the top layer were kept constant.
Fig. 4 Raw HPLC profile from the FXFN module (upper: UV 280 nm, medium: gamma-ray and bottom the pressure).
Harvey (1981) proposed that by adding a large velocity cap layer to a given layered medium and fixing the bottom, the Green's function of full-wave could be approximated with superposition of normal modes of surface waves.
She ended up buying me a white and navy two-piece, with a medium bikini bottom and an extra-large halter top.
A zig-zag arrangement (2 screws on the same level with 1 screw on a lower level between them. Three (or two) rows of screws, with medium screws on the top and bottom rows and small in the middle (or vise versa).
Lower the heat to medium and cook until the bottom is golden brown, 10 to 20 minutes, shaking the pan frequently so the potatoes don't stick and adding oil as needed.
Here, we further develop a general solution of the Hvorslev (1951) formula that accounts for flow in a bounded medium and assumes that the bottom of the river is a prescribed head boundary.
The cells were suspended in 0.3% top agar containing optimum medium and layered onto 0.6% bottom agar made with the same medium.
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