Sentence examples for medium and adjusted from inspiring English sources

Peripheral blood mononuclear cells (PBMCs) were washed, resuspended in culture medium and adjusted to three cell concentrations (1×107/ml, 5×106/ml and 2.5×106/ml).

S. Typhimurium was grown in LB medium to an OD600 of approximately 2, washed by centrifugation, re-suspended in cell culture medium and adjusted by dilution to provide a MOI of 100∶1 or 1∶1 Salmonella to host cells using a conversion of approximately 3×108 bacteria/ml/OD600.

E. coli were grown in LB medium to an OD600 of approximately 1, washed by centrifugation, re-suspended in cell culture medium and adjusted by dilution to provide a MOI of 100∶1 E. coli to host cells in culture wells of a 24-well plate.

Overnight culture of P. aeruginosa PAO1 was harvested by centrifugation at 3000×g for 3 min, the supernatant was discarded and an equal volume of SH medium was added to resuspend the pellet, followed by centrifugation again at 3000×g for 3 min. The pellet was suspended in SH medium and adjusted to a final concentration of approximately 1×109 CFU/ml.

The plasma was centrifugated at 200 g at 4°C for 5 min. After washing three times with 10 mM PBS, the red blood cells were suspended in Leibovitz's L-15 medium and adjusted to a density of 2×107 cells/ml.

To determine the effect of extracellular osmotic pressure on the production of these proteins, we next grew V. parahaemolyticus at 37°C in LB medium and adjusted the osmotic pressure from 0.1 M to 0.5 M by adding NaCl (Fig. 1B).

Imaging had to be interrupted for changing medium and adjusting to the best focal plane and therefore, the data provided from time-lapse observations are often a compilation of several captures.

The initial medium pH was adjusted by 1.2 M HCl or 1 M NaOH to 6.5 ± 0.3 before autoclaving and was slightly increased to 7.5 ± 0.5 after autoclaving, then pH of culture medium was measured and adjusted to 7.0 8.0 every 4 day.

In Canada, fees are based on the storage capacity of a medium and are adjusted to reflect, at least by the Copyright Board's estimate, how often a particular medium is used to record music as well as the overall rate of private music copying.

Standard and adjusted medium was prepared as described above.

The business is targeting in excess of 40% revenue growth in the medium term and longer term, and adjusted EBITDA margins of 35% or above.