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hADSCs were obtained from patients undergoing elective surgery, and then cultured in expansion medium alone or in the presence of FGF-2 (10 ng/ml).
Cryopreserved CB aliquots were thawed, depleted of monocytes, and cultured in serum-free medium alone or serum-free medium with anti-CD3 and interleukins 2, 7, and 12 combined with antibody/cytokines for 48 hours.
MLN cells were cultured for 72 hours with medium alone or antigen.
Wells containing medium alone or phytohaemaglutinin (PHA-L, Sigma Chemicals) were used as the negative and positive controls, respectively.
The lower chamber was either supplemented with DMEM-N2 medium alone or with DMEM-N2-containing 100 ng/ml CXCL12.
Positive controls were cells stimulated with ConA (1 µg/ml), and stimulation with medium alone or with the appropriate % of DMSO were the negative controls.
In these experiments, M were cultured overnight in 96 well-flat bottom plates with culture medium alone or MSC-conditioned medium (50% V/V) (Sn).
C8 did not significantly inhibit the IL-1β-stimulated nitrite secretion in chondrocytes cultured in DMEM medium alone or supplemented with 0.1% BSA (Fig. 5A, B).
Total, and CD4- or CD8-blocked splenocytes [33] were stimulated in vitro with medium alone or with rgp63 (5 µg/ml) for 96 h.
The third and fourth samples were placed back into culture with either DC growth medium alone or DC growth medium plus a maturation stimulus (LPS or DH5α bacteria).
When cultured in hepatogenic differentiation medium alone or co-cultured with Huh-7 cells in differentiation medium, aMSC and more importantly pMSC expressed αSMA at protein level.
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