Sentence examples for medium alone as from inspiring English sources

For determination of cytokine expression and multi-functional T cells by intracellular staining (ICS), splenocytes were cultured with Ag85B-ESAT-6 (5 µg/ml) or medium alone as well as anti-CD28 and anti-CD49d antibodies (BD Pharmingen) for 1 hour, before the addition of Brefeldin A and monensin (Sigma).

Analysis of G6PI-specific cytokine-producing T cells was performed after culturing for 6 h with 25 µg/mL G6PI or medium alone as described.

In the current study, a cellularized scaffold was generated by concurrent deposition of electrospun biodegradable elastomer (poly ester urethane)urea, PEUU) and electrosprayed culture medium + skeletal muscle-derived stem cells (MDSCs) or electrosprayed culture medium alone as a control.

Cells were stimulated in each well with medium alone (as nil control), phytohaemagglutinin (as positive control) and different peptide panels from the specific MTB antigens ESAT-6 (panel A) and CFP-10 (panel B).

Results were expressed as percentage of viable cells, considering control cells incubated with medium alone as 100% viable.

Concanavalin A (Con-A from Canavalia ensiformis, Sigma-Aldrich) was used at 5 μg/mL as a positive control of stimulation, and medium alone as a negative control.

Consistent with the MHEC adhesion molecule expression patterns, degranulation supernatants from WT mast cells increased neutrophil adhesion to WT MHEC monolayer more than twofold over those treated with medium alone, which was as potent as the recombinant TNF-α alone positive control (Figure 3).

Cells were then incubated in triplicates with either N SRS (10 µg/mL final concentration) or, as negative control, with medium alone or, as positive control, with 10 ng/mL PMA (Phorbol 12-myristate 13-acetate, Sigma) and 1 µg/mL Ionomycin (Calbiochem).

PHA (10 μg/ml) was included as a positive control, and medium alone served as the negative control.

Wells with medium alone served as background; Concanavalin A (2.5 µg/ml; Sigma-Alrich, St . Louis MO) was used as a polyclonal stimulator; and known HLA-restricted peptides from Dengue serotype 3 were included in each assay as positive controls.

Spleen cells were stimulated with 5  μg/mL of Concanavalin A Con A (Sigma), employed as positive control, 5  μg/mL of recombinant protein, or culture medium alone, used as negative control.