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Preliminary experiments with cytotoxic concentrations of free BLM revealed that the antibiotic must remain in the culture medium all the time; its removal 3 h after application did not result in cell deaths.
Following a positive selection in minimal medium containing galactose (on solid agar plate) and a second positive selection with medium containing kanamycin (liquid medium), all the clones analyzed displayed the correct targeting (Fig. 2b).
Don't stick to one medium all the time; as a good and open-minded artist, be happy to experiment with whatever you can get your hands on.
Thanks to the decentralized nature of the medium, all the heavy organizational lifting is done by the (often self-authorized) communities that spring up in the wake of the leader's political intervention.
In serum-containing medium, all the FFSNPs had acquired a negatively charged surface (−12.5 ± 3.5 to −18.8 ± 3.4 mV).
For preparation of TYA medium, all the chemical components were dissolved in rice straw hydrolysate (carbon source) and transferred into 125 mL serum bottles.
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NIH3T3 was routinely maintained in a DMEM medium, while all the other cell lines were cultured in a 1640 medium.
Mr. Sitney continued, "In the entire history of the medium, when all the pop-culture interests have faded, a hundred years from now, he will be considered the preeminent artist of the 20th century".
"He personalized the making of music and turned it into an intensely expressive medium with all the nuance of the human voice.
Technorati's State of the Blogosphere in 2009 gushes about the medium with all the pep of Mary Poppins.
The IRC node then publishes this information on its broadcast medium, where all the autonomous agents are listening.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com