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Medium alkalinization experiments were performed as previously described (Felix et al., 1993; de Jonge et al., 2010).
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Induction of medium alkalinization in the plant cell cultures after exposure to the bacteria was measured as described [50].
Does the medium alkalinization by urease-positive cells has an effect on the cell bioenergetics of urease-negative microorganisms sharing the same environment?
ATPases also acts as a possible target of Ca++ activated protein kinase in tomato that is induced by medium alkalinization upon pathogen invasion and wounding [31].
DAG rapidly converts into phosphatidic acid (PA), thus promoting medium alkalinization and triggering downstream MAP kinases and calcium-dependent protein kinases [40].
PTI may include induction of medium alkalinization [21], production of reactive oxygen species (ROS) such as superoxide and hydrogen peroxide, activation of mitogen-activated protein kinases (MAPKs) [22] and callose deposition to reinforce the cell wall at sites of infection [23].
Interestingly, both mutants still prevented chitohexaose [(GlcNAc 6]-induced medium alkalinization.
Since UV-B irradiation of C. roseus cells caused alkalinization of the medium, we investigated whether suramin could inhibit the UV-B-induced medium alkalinization.
(A ) Prevention of chitin-triggered medium alkalinization in the tomato cell suspension assay by Ecp6 mutants.
As expected, this mutant protein was able to prevent chitin-triggered medium alkalinization.
T-test analyses have been now performed on all the alkalinization experiments.
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