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Randomly selected transformants were isolated on selective minimal medium agar.
To solidify the medium, agar (2%) was added.
Aliquots were withdrawn from each generation and plated on LB medium agar and replica plated on LB medium agar containing kanamycin (5 μg ml-1).
In these assays 5 wells were made in minimal medium agar plates.
The transformant, E. coli BL21 DE3)/pET-32a-EG, was selected with LB medium agar plate with ampicillin.
Butanol was then provided at 0.1%v/v, incubated overnight before the bacterial culture was diluted and plated onto LB medium agar to obtain single colonies.
The T. virens ZY-01 was inoculated on Czapek medium agar plate and incubated at 30 °C for 2 3 days.
The first one involves overlaying a solvent onto a medium agar plate or slant which was previously inoculated with bacteria colonies (Li et al. 1998).
The microbial strains were cultured for 48 h using Yeast Peptone Glucose (YPG) medium agar plates (C. albicans) and tryptic soya agar plates (M. Luteus and S. aureus).
We were successful in isolating yeasts that degrade biodegradable plastics from the phyllosphere on minimum medium agar plates containing oil and emulsified PBSA.
After incubation at 37 °C for 2 days, the colonies of strain WZN-1 on LB medium agar plates were approximately 1 2 mm in diameter.
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