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Fig. 4 Number of cells removed from the biofilm due to the washing and medium addition procedures.
An experimental design (2k) was used to determine statistical significance of liquid medium addition and initial pH value.
Immediately after, in half of the wells, the newly added TSB0.65 %G, together with the cells released from the biofilm due to the shear forces exerted by medium addition, was transferred into empty sterile wells.
In the 2nd CM medium, addition of ethanol after the 24th hours decreased the cell density until the 72nd hours and remained stable until the end of the measurement.
Hence, in order to verify the influence of both washing and medium addition procedures in the initial quantity of cells in the biofilm bulk fluid, sequential washes were performed in the same biofilm, and the number of total and cultivable bacteria was determined in the saline solution obtained after each wash.
Infection was performed by filtering the virus containing medium, addition of proteamine sulphate, and transferring of the virus containing medium to the target cells which were incubated further for 24 hrs.
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Non-woven HYAFF flat sheets were seeded with porcine aortic smooth muscle cells (SMCs) and cultured for 14 or 28 days with standard medium or medium additioned with SA.
Addition of GM-1111 thethe medium in addition to LL-37 significantly reduced IL-8 production by cultured keratinocytes (Figure 4B).
When Ang2 was included in the medium in addition to Dll4, a further 7-fold increase in the numbers of proliferating precursors was obtained (Figure 1A).
To increase trypanosome yields we designed an optimized culture medium by addition of supplemental compounds.
F. proliferatum did not produce β-glucosidases in glucose or cellobiose based medium without addition of urea (Table 2).
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