Sentence examples for medium added with from inspiring English sources

A. tumefaciens was grown at 25 28°C in LB medium added with 25 μg/mL kanamycin and employed for the transformation of A. thaliana.

Cells were treated with serum-free medium added with a DMSO solution containing β-glucans at concentrations of 0, 1, 5, 25 μmol/L for 12 h, and then exposed to 10 μg/mL LPS for 40 min.

In culture controls the second stage suspension cultures were added with 100 µL of methanol instead of the drug solution while the drug controls composed of sterile growth medium added with the drug solution.

After approximately 50 generations, the culture was diluted and spread on YES medium added with or without glucosamine, respectively.

Two days after transfection, cell culture was added with 500 µg/ml G418 and maintained under selective conditions for three weeks to allow plasmid integration in the IMR-32 genome; then, survived clones were isolated and expanded in 48-wells plateswithculture medium added with 400 µg/ml G418, then expanded in 24-wells and subsequently in 6-wells plates.

LB medium added with 1% olive oil emulsion was used as enrichment medium.

The next day, the medium was removed and fresh medium was added with the treatments: 25 nM of androstenedione (AD) or AD with 5 nM letrozole (Let) in the absence or presence of GP88 at the indicated concentrations.

The cell culture medium was aspirated and fresh medium was added with reduced serum (1% FBS) and treated with MSA for 24 h.

After four days cells were maintained in medium containing FCS alone, with fresh medium added daily.

Cells were incubated for 2 weeks at 37°C with fresh medium added approximately every 72 h.

The cells were maintained in this medium for 4 7 days, with fresh medium added every second day.