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MiR-17 and miR-20a belong to the medium abundant microRNAs in native USSC and they are found among the most strongly downregulated microRNAs in XXL-USSC, together with miR-106b.
For the low and medium abundant transcripts, we found these two data types correlating well, with Spearman's rank correlation coefficients of ∼0.8.
Since the protein samples are very complex and the high and medium abundant proteins did not seem to be affected by the treatment, a reduction of the complexity could be considered.
Other limitations to the DIGE procedure include a bias towards detecting high and medium abundant proteins and an inability to quantitate proteins that are insoluble or do not stain (see review [ 19]).
The analytical performance of 96FASP with approximately five peptides per protein group was in the expected range given that the stochastic sampling of proteomes with only a few highly abundant proteins by LC-MS/MS generally decreases peptide identifications per protein for many low and medium abundant proteins.
Due to the above mentioned limited predictability of the fingerprints, the method was yet tested only with a limited number of experimentally determined fingerprints and was able to detect differences in gene expression of transcripts representing 0.05% of the total mRNA population (e.g. medium abundant gene transcripts).
Similar(54)
Besides, there are several medium-abundant clusters which are deduced to encode eight novel types of scorpion venom peptides [see Additional file 1].
Pleasures, small and medium, are abundant.
Tumour cells in the control medium displayed abundant cell-associated matrices after 72 h of culture.
In contrast, analysis of conditioned medium revealed abundant accumulation of an N-terminal fragment of ~95 kDa.
The level of TN-C was minimal in the concentrate derived from the 3 hr α-thrombin stimulated conditioned medium, but abundant in the concentrate derived from the 12 hr α-thrombin stimulated conditioned medium.
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