Sentence examples for medium a total of from inspiring English sources
Flux balance analysis shows that, in YE medium, a total of 57 reactions involving NADPH have fluxes (Additional file 5).
To compare SE maintenance on each basal medium, a total of 15 SEs were combined into a group (replicate), with four groups per dish and three dishes per medium treatment (i.e., 12 replicates).
Excluding genes differentially expressed between LNCaP/Co and LNCaP/IL-6#1 cultured in the standard medium, a total of 223 genes were found to be differentially expressed in LNCaP/IL-6#1 at least 2.0-fold greater or 2.0-fold less than those of LNCaP/Co under an androgen-deprived condition.
Autophagic sequestration was blocked by using the purine derivative, 3-methyladenine (3MA) (Enzo Life Sciences/Biomol International, BML-AP502), or the specific phosphatidylinositol (PtdIns) 3-kinase inhibitor, wortmannin (Enzo Life Sciences/Biomol International, BML-ST415) as described., Cells were treated with 5 mM 3MA or 50 µM wortmannin in normal growth medium (a total of 5hrs each).
For transfection experiments, 3 µg of plasmid DNA and 10 µl of Cellfectin (Life Technologies) were combined in 200 µl of serum-free Grace medium (Life Technologies) for 30 min. Immediately prior to transfection, 3×10 cells in 800 µl of Grace medium were prepared and incubated with the transfection medium (a total of 1 ml culture) overnight in a 24-well suspension culture plate (Sarstedt).
Then 20 µl of cell suspension was spotted onto FA medium and a total of 10 spots were added on each plate.
Then 20 µl from the cell mixture was spotted onto V8 agar medium and a total of 10 spots were added on each plate.
Mitochondria were incubated in assay medium for a total of 8 min.
Mitochondria were incubated in medium for a total of 10 min and treated with HNE for 9 min (where appropriate).
Sphere formation to further increase the fraction of cancer stem cells was induced by suspending CD44v3highALDH1high 4T1 cells in 1 1 Matrigel/basal medium in a total of volume of 100 μl.
After the final selection, we pooled the strains and cultured them alongside a negative control strain (ura3 Δ:: natMX) in glucose- or galactose-containing medium for a total of 20 generations.
