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Cytokines and mediators were analyzed by ELISA and RIA techniques.
Blood samples were obtained and plasma concentrations of an array of inflammatory mediators were analyzed.
Correlations between fgf21 expression and mitochondrial respiratory chain enzyme complex activities and ISR mediators were analyzed.
Mediators were analyzed by ELISA (N = 27), immunoassay (IA) (N = 8), Luminex multianalyte technology (LMAT) (N = 1), Bio-Plex human cytokine assay (BHCA) (N = 1), custom protein array (CPA) (N = 1), or Quantibody Ar kit (QAK) (N = 1).
All mediators were analyzed at room temperature (i.e. 23°C).
Alternatively, cells were cultured for 24 h; and secreted mediators were analyzed in culture supernatants.
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A specific set of IIR mediators was analyzed.
It is more likely that identifying promising biomarkers will only be possible when a number of mediators are analyzed as a profile [ 204, 205].
Thus, the effect of HSF on the expression of H3K4me3 and H3K9/14Ac at the promoters of the previously mentioned inflammatory mediators was analyzed.
Moreover, effect of HSF (Sun et al., 2014) on toll-like receptor (TLR -induced upregulaTLR -inducedme3 and H3K9/14Ac expression, and increased Pol-II recrupregulationhe profoter of tH3K4me3mune mediandrs was analyzed.
To confirm these observations, the activation of caspase-3/-7, a key apoptotic mediator, was analyzed in A431 cells treated with GBT for 24 h.
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factors were analyzed
intermediates were analyzed
mediators were analysed
mediators were assessed
mediators were brought
mediators were increased
mediators were entered
mediators were eluted
mediators were associated
mediators were related
mediators were surprised
mediators were correlated
mediators were quantified
mediators were called
mediators were produced
mediators were changed
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