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Mediation was tested using the guidelines provided by MacKinnon and Dwyer (1993) and MacKinnon, Fairchild, and Fritz (2007): we tested the effects of the independent variable to the mediator (path α) and the effect of the mediator to the dependent variable when the independent variable was taken into account (path β).
To formally test the mediation effect as described in the third step above, we will use a version of the Sobel test [ 41], which tests whether the indirect effect of intervention on the outcome through the mediator (defined as the product of the intervention to mediator path and the mediator to outcome path) is significantly different from zero.
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Furthermore, the relationship between shift work and the MetS did not change when taking into account all three suspected mediators (path c'), and therefore no mediation is apparent.
For each potential mediator, single-mediator path models in three causal conceptualisations were considered.
A diagram of the multiple-mediator path model is shown in Figure 2.
Results of the single-mediator path model are shown in Table 2.
> -wrap-foot> aEstinathe in the multiple-mediator path model; bEstimated in the multi-group path model; CI = Confidence interval.
According to the Baron and Kenny criteria, mediation occurs if: (1) the independent variable shift work affects the dependent variable MetS (path c); (2) the independent variable shift work significantly affects the mediators (path a); and, (3) the mediators affect the dependent variable MetS while controlling for shift work (path b).
In this so-called product of coefficient test, the product of coefficients from the independent variable to the mediator (a path in Fig. 1) and the coefficient from the mediator to the dependant variable adjusted for the independent variable (b path in Fig. 1) is divided by the standard error of the product to create a test statistic.
In the mediation analysis of treating multiple sexual partnerships as a potential mediator in the path of RDU and HIV infection, "multiple partnerships" was independently associated with both RDU (aOR, 1.69; 95% CI, 1.44-1.98) and HIV infection (aOR, 1.35; 95% CI, 1.06-1.71).
Multiple partnerships served as mediator on the path of RDU and HIV infection (Table 2).
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