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To calculate the proportion of the association between early menarche and type 2 diabetes risk that is mediated by adult BMI, we performed mediation analyses in the InterAct subcohort using methods previously described for dichotomous outcomes (25, 26).
Moreover, when we used mean regression to conduct mediation analyses in the same cohort (with air pollution as exposure, methylation as mediator, and cardiovascular-related blood markers as outcomes), we estimated a positive indirect effect of PM2.5 black carbon on fibrinogen through a decrease in F3 methylation (Bind et al. 2014).
Similar to Study 1, we conducted mediation analyses in accordance with Baron and Kenny [ 24].
Researchers are advised to undertake mediation analyses in more than one balanced dataset [ 24].
This article presents a guide to important ways in which the use of mediation analyses in child and adolescent treatment outcome research can be improved.
Furthermore, mediation analyses in this trial will indicate potential mechanisms of action which could be the target of future efforts to enhance FBT.
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This is of particular concern in mediation analyses, if either of the mediators (in this case GGT) is a good proxy for the true exposure, leading to an inflation of the estimated indirect effect.
For all mediation analyses shown in the Additional file 1: Table S3 and Additional file 1: Table S4 the overall findings remained essentially unchanged.
The mediation analyses conducted in this study suggest that approaches targeting fruit liking may have only limited impact on fruit consumption, although the conditions under which liking may influence behaviour merit further investigation.
Similarly, causal diagrams focused our attention on the structures of oft overlooked potential biases, such as biases due to time-dependent confounding in stratification-based analyses [ 12], mediator-outcome confounding in mediation analyses [ 13], selecting on treatment in instrumental variable analyses [ 14], and naïve per-protocol restrictions in randomized trial analyses [ 15].
All mediation analyses were implemented in the M3 Mediation Toolbox (http://wagerlab.colorado.edu/files/tools/mediation.html) (54) in Matlab 7.11.
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