Sentence examples for mediating transgene expression from inspiring English sources

BacMam virus, a modified baculovirus mediating transgene expression under the control of a mammalian promoter, has emerged as a versatile and safe vector during vaccine development.

The expression levels of the minicircle groups were significantly higher than those of the corresponding parent groups, demonstrating that the minicircle is more efficient in mediating transgene expression in vitro.

By functionally rewiring luteinizing hormone receptor signaling to CREB1 (cAMP-responsive element binding protein 1 -mediated transgene expression via the common cyclic adenosine monophosphate (cAMP) second messenger pool we have designed an artificial insemination device which enables lutropin-triggered in-utero release of sperms protected inside cellulose-based implants.

To determine whether the lentiviral vectors could efficiently mediate transgene expression in mouse skeletal muscles under the control of the MSCV promoter, we injected 5 µl of LvMSCV-EGFP vector into the TA muscles of 3-day-old C57Bl/10 mice.

The AHA promoter-mediated transgene expression in the neonatal liver was about 5-fold lower than the CMV promoter-mediated transgene expression.

While efforts continue to improve upon the efficiency of these regulatory systems, we have focused our attention to developing a transcriptionally regulated oncolytic HSV-1 system that mediates transgene expression in a cell cycle-dependent manner.

It is well-documented that the small intestine displays cell signaling across its defined epithelial and mesenchymal cellular compartments [ 2, 12- 14], offering an ideal system to display lentiviral-mediated transgene expression to modulate mesenchymal-epithelial crosstalk.

After normalizing for the activity obtained with the CMV driven plasmid it could be observed that the AFP positive cell lines HUH7 and HepG2 were most susceptible to AFP mediated transgene expression, whereas in almost all other cell lines the hCMV enhancer element just boosted basal expression levels in general.

These cell cycle regulatory elements have facilitated the ability of YE-PC8 viruses to mediate transgene expression in accordance with the proliferation status of the host cells, but this was not observed in matched control virus where the luciferase reporter gene was placed under the regulation of the ubiquitous CMV promoter.

However, Adv-mediated transgene expression is transient, and it causes dose-dependent toxicity [24] and activation of host immunity [25].

A more recent study of Agrobacterium-mediated transgene expression in apple utilized Rubisco small subunit gene promoters from tomato (RBCS3C) and soybean (SRS1) to drive expression of GUS [ 8].