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Transposons as a type of MGE are polyphyletic with respect to the enzymes mediating their transposition [ 10, 13, 15].
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Most TEs encode proteins that mediate their autonomous transposition.
Generally, two different TE classes are distinguished by their transposition intermediate: RNA or DNA.
§TEs have been divided into two classes based on their transposition intermediate.
Plasmids belonging to the IncA/C incompatibility group are of interest as they carry resistance to diverse groups of antimicrobial agents and have a broad host range [ 17]. blaOXA-181 has been found as part of transposon, Tn 2013, with upstream insertion sequence IS Ecp1 mediating one-ended transposition of the blaOXA-181 gene [ 16].
TEs are generally classified according to their transposition mechanism.
This is believed to suppress most of their transposition activity [9], [10].
Several DNA transposons in newly synthesized Arabidopsis allopolyploids, also display transcriptional activity, although their transposition is limited [34].
SINEs are too small to harbor a coding function, and for their transposition they use reverse transcriptases encoded by LINEs.
These ISs use REPs as targets for their transposition.
The transfected cells were subjected to a chromosomal transposition assay (as detailed in reference 32) to determine their transposition activity.
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