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We combine these three approaches with an ethnographical analysis to achieve a deeper understanding of several difficulties with pTD that are typical in the realization within the AAL field, especially when a mediating method, such as SBD, is adopted.
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For additive transgenesis a novel transposon mediated method, e.g., the Sleeping Beauty transgenesis, increased the efficiency, and its application to create cardiac disease models is expected in the near future.
In Ref. [52], Au NRs were first synthesized by a two-step seed mediated method.
Further to this, a novel whole-cell mediated method was developed; using a model Fe(III -reducing bacterIII -reducing an Fe(III) oxyhydroxide, producing nano-scale magnetite with a narrow size distribacteriumto contreducele reantivity and particle size [27].
We synthesized the pyrazolyl thiourea derivatives using rapid ultrasound mediated methods and confirmed their structures by NMR and IR spectra.
Prior research focuses on learning enhancement from several perspectives like computer mediated methods, learning games, personalized learning environments and web based instructional design.
There is also an emphasis on using microbes for the synthesis of nanoparticles as compared to chemical methods since the microbe mediated methods are environmentally benign and sustainable (Sharma and Mudhoo 2010; Singh 2015; Virkutyte and Varma 2013).
> For this discussion, the methods are broadly classified into biologically, chemically, and physically mediated methods.
The work highlights the possibilities for optical injection as a realistic alternative to other injection techniques such as electroporation, microinjection, ballistic particle insertion (gene gun), or chemical or viral mediated methods.
The bombardment and protoplast methods can overcome the host-dependent shortage of the Agrobacterium-mediated method.
The Agrobacterium-mediated method is the easiest and most convenient method.
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