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However, in contrast to the YaeT proteins, the TpsB proteins do not form heterooligomeric complexes and while YaeT is promiscuous for OMPs in general, mediating insertion of OMPs into the OM, TpsB proteins are selective for their genetically linked TpsA protein and allow for translocation across the OM [58].
Taken together, these data establish that the GET system is directly responsible for mediating insertion of newly synthesized TA proteins into the ER membrane.
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After we have demonstrated the twin ribozyme mediated insertion of additional nucleotides into a predefined position of a suitable substrate RNA, we here show that a similar type of twin ribozyme is also capable of mediating the opposite reaction: the site-specific removal of nucleotides.
The HAP1-KIF5 couldex could mediate insertion of GABAA receptors into the surface membrane at synapses [ 88].
Regardless of the underlying mechanism, however, this integration event already provides evidence that TALEN-induced cleavage can mediate insertion of alternative sequences at TALEN sites in vivo.
Using LoxP-targeted, Cre recombinase-mediated insertion of an L4 cassette to displace GFP from the regulated promoter in this parent clone, cell line A1-L4 was generated.
To facilitate the analysis of linker mediated insertion site sequences, we have developed a web application called iMapper (Insertional Mutagenesis Mapping and Analysis Tool).
Agrobacterium VirD2 also helps mediate insertion of this end into target DNAs [38], [43]; the corresponding relaxases of transferable plasmids probably mediate recircularization of transferred (linear) plasmid DNA in recipient cells [44].
(5) Ng-mediated insertion of GluR1 is dependent on CaM CaMKII interaction, which results in CaMKII activation.
In vitro reconstitution demonstrates that the Get proteins directly mediate insertion of newly synthesized TA proteins into ER membranes.
jsIs1269 was created by MosSCI-mediated insertion of NM2645 into the ttTi14024 Mos insertion site on the X chromosome using the direct integration protocol (Frøkjær-Jensen et al., 2012).
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