Sentence examples for mediating excision of from inspiring English sources
Orientation of insert DNA post-recombination is dependent on the orientation of the sites prior to the reaction, with sites in the same orientation on a given DNA strand mediating excision of intervening sequence and sites in opposite orientation mediating inversion of intervening sequence.
These loxP sites allow Cre recombinase [33] to mediate excision of 'lacZ and kan either in vivo or in vitro.
We generated a transgenic mouse that overexpresses Drp1 after Cre recombinase-mediated excision of floxed nuclear DsRed sequence.
This strain expresses the Tomato transgene only upon Cre mediated excision of the floxed-translation Stop sequence in the Rosa26 locus.
However, Ramirez and colleagues showed that the integrase was able to recognize and mediate excision of a truncated site associated to aadA1, indicating that the genetic context of such truncated sites could influence their role in IntI1 recognition and mobilization.
The many-faceted SOS network typically starts with induction of the UvrABD complex, which together with UvrC (which contains two endonuclease domains), mediates excision of nucleotides from damaged DNA [ 7].
The Bm variable genes are found mostly in contiguous regions flanked by insertion sequence (IS) elements, which appear to mediate excision and subsequent elimination of groups of genes that are under reduced selection in the mammalian host.
Use of a demonstrated recombinase expression line such as COXS3-9.17 COXS3-9.17fficienabledombinasufficientd excision events to excise all tarecombinaseen crossed together.
The system has been successfully shown capable of recombinase mediated excision, inversion and integration reactions.
Use of a demonstrated recombinase expression line such as phiC31.31.83 (Table 3) enabled sufficient recombinase mediated excision events to fully excise all target DNA when crossed together.
We included loxP sites to enable reversion by Cre-mediated excision of the SAIRESβgeo cassette.
