It was suggested that vasopressin accelerates extinction when given after acquisition by reducing the effectiveness of LiCl and it prolongs extinction when given before acquisition by altering neural responsiveness in areas mediating conditioned taste avoidance.
Our findings were concordant with previous studies [ 6, 8, 18] on antisense transcription in yeasts and they also highlighted the importance of antisense regulation in mediating condition-specific transcription in yeasts.
The specific mechanisms underlying LHA ghrelin mediated feeding will require further studies beyond the scope of this manuscript, but we believe that this additional experiment adds further support for the importance of vHP GHSR-1A signaling specifically in mediating conditioned feeding effects and improves the overall quality of the manuscript.
Instead, it has been assumed, by analogy to circuits mediating conditioned defensive responses, that emotion centers for innate defensive behaviors would be located in the medial amygdala (MEA), and that downstream hypothalamic targets would similarly serve as passive relays for amygdala output (Gross and Canteras, 2012; LeDoux, 2012; LeDoux and Damasio, 2013).
After consumption of a novel sucrose solution, temporary cooling of neural areas that mediate conditioned taste avoidance can itself induce conditioned avoidance to the sucrose.
If they are involved, then one would expect that cooling locations in the brain that do not mediate conditioned taste avoidance, such as the caudate putamen (CP), would induce conditioned taste avoidance as long as the meninges were cooled as well.
Dopamine neurons also mediate conditioned ethanol preference.
As specifically suggested by Reviewer #2 we have added an additional experiment which compares the relative importance of hippocampal GHSR mediated conditioned feeding with another GHSR rich region, the lateral hypothalamus (LHA).
Researchers studying other single genes have found evidence for recent evolutionary change in the genes that mediate conditions like skin color, resistance to malaria and salt retention.
This might reflect more ready symptom reporting rather than more prevalent IgE-mediated conditions 12.
