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However, when it was used solely for mediating a bicistronic expression (pZCpTG), it was efficient in the primary cells and embryos.
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We also constructed a 2A peptide mediated bicistronic expression vectors for RFP and Lhx1, verifying simultaneous expression of RFP and Lhx1 (Trichas et al., 2008) (supplementary material Figs S1B, S2).
We generated a bicistronic construct including a second-generation CAR and a truncated-epithelial growth factor receptor marker.
In this study, an optimized eukaryotic expression plasmid was developed and an optimized DNA vaccine co-encoding antigenic and adjuvant peptide using a bicistronic expression system was designed.
The Streptomyces tyrosinase is encoded by a bicistronic operon, melC.
E-RTA is coordinately expressed as a bicistronic RNA transcript with EBV-Z [37].
Non-cleavable MKK mutants were cloned into a bicistronic CGW-IRES-EGFP vector [39].
This result indicates that a bicistronic pcm-nlpD message is indeed transcribed from Txn1.
Unc119 overexpression by a bicistronic GFP-RV-Unc119 retrovirus reduced bacterial uptake by 40% (Figure 5A).
The current study employed a bicistronic luciferase reporter system to monitor suppression of a series of missense mutations.
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