which mediates a homologous recombination event between the FRT sites from the HEK 293 Flp In cell genome and the pcDNA5/FRT vector.
We propose that the spontaneous deletion and mobilization of vector sequence from the recombinant adenoassociated virus (rAAV) integration locus is mediated by a recombination event between the inverted terminal repeats that define the boundaries of the individual genome subunits.
After Cre-mediated excision, a recombination band appears when primers flanking both loxP sites are used with the same DNA samples (Fig. 1D).
Sequence alignments and breakpoint analysis of the 2DL5-2DS35 gene block indicated that the first step in this transfer may have been mediated by a recombination event between the intron 2B regions of 2DL5A in the telomeric segment and 3DP1 from the centromeric region.
The integrase PhiC31 mediates the recombination between an attP site in the genome and an attB site in a targeting vector.
We therefore inferred that REs may have played an important role in this dispersed gene duplication by creating the requisite homologous DNA sequence that mediate a DNA-level recombination, most probably by a non-allelic homologous recombination (NAHR) mechanism.
The unique somatic diversification of receptors of the immunoglobulin family during ontogeny [ i.e., V(D J recombination] mediates a dramatic increase in the number of foreign pathogen epitopes that the adaptive immune system can remember and attack (Janeway et al. 1996; Zhu et al. 2012).
RecA family proteins, including bacterial RecA, archaeal RadA, and eukaryotic Dmc1 and Rad51, mediate homologous recombination, a reaction essential for maintaining genome integrity.
Also, transposable or other known repetitive elements are not likely to mediate a link between recombination hotspots and microsatellites in yeast, since these elements are not enriched in yeast hotspots [ 42], as they are in human hotspots [ 43].
These results led Tobiasson and colleagues to propose that Piv invertase and the IS110 transposases could mediate DNA recombination by a common mechanism involving a catalytic DED or DDD motif [ 27].
