The remaining hydrogen peroxide in the reaction mixture mediates a second reaction with a chromogenic reagent to a quinoneimine dye coupling product measuring at 520 nm.
After introducing streptavidin-conjugated alkaline phosphatase (SA-ALP), the specific binding between streptavidin and biotin mediates a catalytic reaction of ascorbic acid 2-phosphate (AA-P) substrate to produce a reducing agent ascorbic acid (AA).
bcHIBADH mediates a dehydrogenation reaction on S-3-hydroxyisobutyrate substrate with high catalytic efficiency in an NAD+-dependent manner; it also oxidizes l-serine and 3-hydroxypropionate with lower activity.
Amino acid sequence analysis shows that CqsA bears homology to PLP-dependent acyl-CoA transferases, and previous work has shown that CqsA mediates an acyltransferase reaction between LAB and d-CoA to produce Am-CAI-1.
Selective recognition of Hg2+ ion in aqueous solution was mediated through a reaction-based (Kucherov reaction) approach.
COX-1 and COX-2 are bifunctional enzymes that mediate a COX reaction whereby arachidonate plus two molecules of oxygen are converted to the cyclic endoperoxide PGG2, followed by a hydroperoxidase reaction in which PGG2 undergoes a two-electron reduction to PGH2 [ 8].
Natural killer T (NKT) lymphocytes mediate a rapid reaction to the glycolipid drug α-galactosylceramide (αGalCer), which triggers release of large amounts of cytokines into the serum within 12 h, starting with interleukin 4 (IL-4).
It is a highly effective catalyst mediating a pivotal reaction in the antioxidant pathway [16].
RecA family proteins, including bacterial RecA, archaeal RadA, and eukaryotic Dmc1 and Rad51, mediate homologous recombination, a reaction essential for maintaining genome integrity.
Then, the PCR products were used as a template for in vitro transcription and the RNase A-mediated cleavage reaction using an EpiTYPER Reagent Kit (Sequenom).
