Nevertheless, our results do imply that cell traction-mediated unfolding of 10FNIII can lead to the formation of partially unfolded kinetic intermediates that can influence the rate of FN fibril formation through several different mechanisms.
These observations are consistent with the notion that cell traction-mediated unfolding of 10FNIII leads to the formation of a partially unfolded intermediate that may facilitate the formation of hydrophobic contacts between different FN domains and drive fibril formation [23], [24].
Fibronectin unfolding revisited: Modeling cell traction-mediated unfolding of the tenth type-III repeat.
The decrease in ellipticity at 295 nm is indicative of UP1-mediated unfolding of the G-quadruplex structure.
Temperature-mediated unfolding of 2G12-IgG as monitored by both ECD and DSC followed a clear non-two-state unfolding pathway with two domains of different sensitivity, representing the Fab and Fc fragments of the IgG molecule [32].
The PA pore functions as a translocase, mediating unfolding and translocation of bound EF and LF across the membrane [2].
Recent simulations of force-mediated unfolding of FN domains and sequences analysis of human versus mouse FN suggest that the structural stability of the FN's central cell-binding domains (FN III9 10) affects its integrin specificity.
In the present case, an extension velocity of v = 0.01 Å/ps was used; this rate is at least fifty times slower than that used in past simulations of force-mediated unfolding of 10FNIII [20], [20].
Consequently, the goal of this study was to uncover molecular details of force-mediated unfolding of the 10FNIII domain under physiological loading conditions by applying tension along an axis that includes the integrin-binding RGD loop.
This suggests they have a native state with an increased thermodynamic stability, consistent with conclusions drawn from denaturant-mediated unfolding of the latter two variants [ 29, 50].
The urea-mediated unfolding of wt BFDC and the R141E/A306F variant was followed by both intrinsic fluorescence and CD spectroscopy.
