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AAV mediated transfer of full length Fto cDNA was used for overexpression; ii).
Thus, rather than manipulating Fto expression in specific ARC neuronal populations, we chose a strategy employing AAV mediated transfer of Fto cDNA and shRNA coupled with stereotactic injection in order to manipulate Fto expression within the ARC (Fig 1C).
Three major lines of evidence support the Khoe-San mediated transfer of the -14010C allele.
Ad mediated transfer of shERα expression constructs results in efficient and specific knockdown of endogenous ERα transcription both in vitro and in vivo.
Reimchen [ 4] documented an average input of 120 kg/ha of N into a riparian zone on Haida Gwaii, largely from black bear mediated transfer of salmon carcasses.
In addition, a high plasma VLDL-triglyceride concentration leads to the generation of smaller, more dense LDL particles through the cholesteryl-ester mediated transfer of lipids between VLDL and LDL particles.
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Valadi, H. et al. Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Satoh, S. et al. AXIN1 mutations in hepatocellular carcinomas, and growth suppression in cancer cells by virus-mediated transfer of AXIN1.
Levin M, Mercola M. Gap junction-mediated transfer of left-right patterning signals in the early chick blastoderm is upstream of Shh asymmetry in the node.
Cyclic AMP sponge was fruitfully applied to examine feedback regulation of gap junction-mediated transfer of cAMP in epithelial cell couplets.
We hypothesize that transplacental and milk-mediated transfer of maternal immune cells and/or their cytokines is essential for normal brain development and for the establishment of adult behavior in the offspring (see figure below).
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