Data show differential recruitment of GFP-ER to the array, with the AF1 domain playing a vital role in EGF-mediated responsiveness.
We conclude that HUVECs produced a soluble inhibitor of LPA-mediated responsiveness, which we tentatively name ILMR.
The observed changes in aphid-mediated responsiveness of aos had, however, no noticeable impact on aphid fitness.
miR expression analysis in doxorubicin treated cells differing for p53 status supported p53-mediated responsiveness for miR-10b, -151a and, limited to MCF7 cells, also -23b.
Moreover, in mammals several C3 cleavage fragments, including C3a has been demonstrated to be linked to the CXCR4-mediated responsiveness of hematopoietic stem cells [ 71].
Downstream of and consistent with the yeast-based results, ChIP assays further supported the putative function of the identified p53 REs in modulating p53-mediated responsiveness of miR genes.
Analyses of reduced data were used to minimise the number of the main hypothesis tests (H0 = infection variables are unrelated to TLR-mediated responsiveness), which were then followed up by exhaustive post hoc testing of individual variables [ 72- 74].
The putative inhibitor, which we tentatively call ILMR (inhibitor of LPA-mediated responsiveness) seemed to act on cells (instead of at the level of LPA) by suppressing the ability of LPA receptor 1 to signal.
Cortisol suppressed IL-6 mRNA levels in dermal (86%; P < 0.01), bone marrow (50%; P < 0.01) and synovial fibroblasts (87%; P < 0.01), indicating that all three cells had a similar capacity for GR-mediated responsiveness.
To validate p53 family-mediated responsiveness of the newly predicted target miRs we first evaluated the potential for wild type p53, p63β and p73β to transactivate from p53 response elements (REs) mapped in the miR promoters, using an established yeast-based assay.
