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Both these events occur several days before Nestin-Cre mediated recombination is complete.

The efficacy of ExoS54-Cre mediated recombination is represented as the percentage of EYFP positive cells in the infected mESC population (Fig. 4D).

Clearly, the proportion of lacZ positive cells increases with the increased number of cells in S phase, thus the Cre mediated recombination is significantly influenced by the chromosome structure.

Although some studies of the mCrem system [20], [24], [36] have suggested that mCrem mediated recombination is tightly controlled in the absence of ligand, work from other labs suggested ligand independent mCrem mediated recombination might occur, albeit at low frequency [11], [19], [26], [27].

Cre mediated recombination is a powerful and broadly utilized genetic approach to conditionally delete or express selective genes, and is extensively used to lineage trace distinct progenitor populations during development (reviewed by Branda and Dymecki, 2004; Bouvier and Cheng, 2009).

Cre mediated recombination was visualized with either the ROSA26R reporter line, which expresses lacZ upon Cre mediated activation or the Z/AP double reporter line [33], [35].

Tie2-Cre mediated recombination was confirmed in extra embryonic blood islands of E7.5 Spry1;TiembryosRosa26LacZ embyyos by β-galactosidase staining (Figure 2A and data not shown).

Cre mediated recombination was induced at the beginning of neurogenesis in a selected cohort of proliferating RG cells that were subsequently traced during forebrain development and in post natal neurogenic niches.

The efficiency of lentiviral-Cre mediated recombination was analyzed by Western analysis and indirect immunofluorescence, demonstrating a 10-fold reduction in ERK2 protein levels (Figure 2A and Figure S2C, respectively).

In contrast to the Nestin-cre line, Emx1 cre mediated recombination was sufficient in the VZ/SVZ of the developing cortex at both E12.5 and E14.5 (Fig. 2B), which we confirmed again using antibody staining for cre (Fig. 2C).

A genome-wide database of primers for the 5' and 3' fragments for construction of the deletion cassette by yeast mediated recombination was generated using a Python script (provided by Peter Andrews of the Neurospora Genome Project) that serialized the function of the Primer3 core primer design program [ 48].