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Pretreatment of the cells for 24 h with 1 mM AICAR did not significantly reduce the mediated rate of apical to basal flux (6.2 ± 1.4 vs. control 4.1 ± 0.7 fmol·cm−2·min−1).
From the previous experiments apical cell entry was shown to be reduced in the AICAR-treated cells; nevertheless in the same cells the mediated rate of [H]-D-Phe-L-Gln apical-to-basolateral transepithelial flux was not reduced.
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Extrapolated literature values for microbial oxidation of Fe(II)(aq) by neutrophilic microbial populations to the simulated reactor conditions suggested that the microbially-mediated rate may be several orders-of-magnitude slower than the surface-catalysed oxidation.
The same acid-mediated rate constant was assumed for both forms A and B (kHA= kHB), since the data do not allow for sufficient discrimination.
For NHε2, three parameters were needed (W, kOHB, and kOHC), whereas the acid-mediated rate constants kHA and kHB could not be determined.
Extrapolation of the linear portion of the plot shown for MCT1 inhibition to zero carrier-mediated rate (i.e. water-injected rate) provided an estimated concentration of MCT1 in the incubation of 40 nM.
The results of this study suggested that the chemical properties of exogenous compounds significantly influence BCRP polymorphs mediated uptake rate.
As detailed above, the hPepT1-mediated transport of [H]-D-Phe-L-Gln into the Caco-2 cell monolayer and the mediated transepithelial rate have been calculated by subtraction of the transport observed in the presence of 20 mM Gly-L-Gln.
It seems likely that stimulation of carotid chemoreceptors prevents the expected baroreceptor mediated heart-rate response.
A few years ago, a model was proposed to predict the effect of the pore architecture of a bone graft substitute on its cell-mediated resorption rate.
The cAMP-mediated sweat rate test may be a useful endpoint for studies of new agents to increase the function of CFTR.
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