Therefore, in Ccel, the CCR-mediated monitoring of cellular needs for energy and the TCS-mediated sensing of environmental substrate-availability likely ensure both sensitivity to environmental nutrients and the efficiency of cellulose degradome.
By contrast, urea-mediated unfolding monitored by fluorescence spectroscopy suggested only one main unfolding event.
We demonstrated the more general applicability of this method to dynamically track receptor-mediated phagocytosis by monitoring internalization of FITC-labeled fibronectin beads in NIH 3T3 fibroblast cells.
Further, we sought to determine which apoptotic pathway(s) are involved in the RRR-γ-tocopherol-mediated apoptosis, by monitoring caspase activation.
For monitoring compound mediated cytotoxicity, cells were then treated with DMSO only or with 0.2 μM, 1 μM or 5 μM olaparib and incubated for an additional 72 hrs.
Bioactivity of the released drug was determined by monitoring Dex-mediated, dose-dependent apoptosis of human peripheral blood derived monocytes in culture.
We verified that the concentration of MG132 used effectively blocked proteasome activity by monitoring ICP0-mediated PML degradation [23], [25], [49] (Figure 3, panel II).
We investigated whether Flv attenuated the ER stress-mediated apoptosis pathway by monitoring CHOP expression and α-caspase-4 processing.
The organoid-culture protocol has been standardized in our laboratory by Moon et al. (unpublished data) for monitoring CFTR-mediated fluid secretion.
Several additional studies have utilized the HaloTag platform for imaging cellular events, including visualization of clathrin-coated pits, monitoring chaperone-mediated autophagy, and investigating peroxisome growth and degradation.
H3 (pHH3) is often implemented for monitoring drug-mediated mitotic changes in clinical trials; however, data on the expression level among different cancers are limited.
