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This research focuses on the development of a fast ultrasonic assisted ferrofluid mediated methodology to obtain the optimum conditions for silver adsorption from aqueous solutions.
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Electroporation-mediated methodologies also were not successful.
Similar to the method described in Wesolowska and Rong (2013), we made use of single-strand annealing (or one-ended invasion crossover) to target genes close to an attP site (in our case the attP is provided in the MiMIC), but we postulate that this strategy can also be used when resorting to the attP-mediated CRISPR/Cas9 methodology.
Finally, the adaptamer-mediated PCR fusion methodology is versatile and can be applied to varied genome manipulations.
Ten genes were further selected to assess their role in the blood-feeding process using RNAi-mediated gene silencing methodology.
Here, we describe a novel methodology to quantitatively analyze nanocarrier-mediated disposition of siRNA.
After successfully knocking out the ssrA gene using the cosmid-mediated recombination methodology, we were able to demonstrate germination, growth, and sporulation defects of ΔssrA mutant at both high and routine growth temperatures.
While ongoing efforts are aimed at defining the optimal conditions and rate of Tol2-mediated BAC transgenesis, our current methodology should have immediate applications in comparative genomic analysis of cis-regulatory elements and gene functions in mice and zebrafish.
Elimination of the dithiocarbamate group gives α,β- or β,γ-unsaturated lactams depending on both the methodology employed (base-mediated or thermal) and the nature of the carbocycle fused to the β-lactam.
Sortase mediated ligation is therefore a straightforward methodology for the preparation of solid supported enzymes and bead based assays, as well as the modification of planar surfaces for microanalytical devices and protein arrays.
As such, CENH3-mediated centromere labeling constitutes a unique methodology to detect and to quantify endomitotic endoploidy in vivo.
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