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The complement mediated lysis of the grafted RBCs was dependent on the type of polymer, grafting concentration and molecular size of grafted chains.
SCs were purified by using cytosine arabinoside and Thy-1.1 antibody (Sigma) mediated lysis of the fibroblasts.
The presence of C4BP in this setting may also prevent complement mediated lysis of the tumour cells.
A T-lymphocyte mediated lysis of MMC treated Tenon's capsule fibroblasts could be responsible for this problem [ 9].
Both total population and CD16+ depleted subsets of monocytes could significantly prevent NK cell mediated lysis of MSCs (Fig. 3A) and DPSCs (Fig. 3B and 3C).
Both total population of monocytes and those depleted of CD16+ subsets were able to prevent NK cell mediated lysis of MSCs and DPSCs, and to trigger an increased secretion of IFN-γ by IL-2 treated NK cells.
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Figure 6: BNP antibody-dependent complement-mediated lysis of cells correlates with MHC class I expression.
Hematopoietic potential and NK cell-mediated lysis of ESC- derived CD45+ cells.
A recent study showed that BNP alloantibodies could induce complement-mediated lysis of MDBK cells18.
MHC I expression is higher on PBMC than on granulocytes and MHC I mAb dependent complement-mediated lysis of PBLk strongly increased the granulocyte/PBMC ratio (Fig. 6c,d), reflecting the killing of PBMC rather than granulocytes.
Pende, D. et al. Analysis of the receptor-ligand interactions in the natural killer-mediated lysis of freshly isolated myeloid or lymphoblastic leukemias: evidence for the involvement of the Poliovirus receptor (CD155) and Nectin-2 (CD112).
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