FAF1 is a 74-kDa protein with an N-terminal domain that mediates its interaction with Fas, the Fas-interacting domain (FID), and a functional ubiquitin-like C-terminal domain that is required for its pro-apoptotic activity.
If Sir2 is not sumoylated, it is presumable that some sumoylated proteins may mediate its interaction with other proteins such as Esc2 and regulate its activity.
Domain I of NS5A, was sufficient to mediate its interaction with NS4B.
The lysosomal localization of AGA is dependent on a conformational determinant that mediates its interaction with the phosphotransferase that generates the M6P signal, a prerequisite for the lysosomal targeting of AGA21.
The SH3 domain of CAS mediates its interaction with several proteins involved in signaling pathways such as focal adhesion kinase (FAK), tyrosine phosphatases PTP1B and PTP-PEST, and the guanine nucleotide exchange factor C3G.
As our polysome-profiling results suggested a role of Xrn1 in translation initiation (Fig. 2b), we examined whether Xrn1 interacts with the eIF4F complex, a key component of the translation initiation machinery that binds to capped mRNA and mediates its interaction with the 43S pre-initiation complex20.
It is now established that the specificity in signaling of signal transducer and activator of transcription 3 (STAT3) is mediated by the SH2 domain of STAT3, which mediates its interaction with the phosphopeptide docking sites displayed by receptors and JAKs, dimerization and subsequent DNA binding.
The Derbyshire lab has previously identified that the C-terminal domain of PfHsp70-1 mediateitse interactiontion with PI3P, which could play an important role in parasite survival.
To identify the domain(s) on HDAC6 that mediates its interaction with CLIP-170, plasmids that express various truncated forms of HDAC6 were transfected together with GFP-CLIP-170.
We transfected Agt5 −/− MEF cells with wild-type p62 or a LIR deletion mutant, which was reported to mediate its interaction with LC3 for autophagic degradation, and found that the protein level of p62 or its LIR deletion mutant were significantly reduced when parkin is expressed in these cells (Fig. 3C).
