In fact, CyaA-mediated intoxication by cAMP was also found to inhibit the phagocytosis of B. pertussis cells via Fc receptors by neutrophils (Weingart & Weiss, 2000).
Comprehending how the needle-tip complex interfaces with the type III machinery and the translocon assembles in eukaryotic membranes will be important towards understanding the T3SS-mediated intoxication mechanisms and protective nature of antibodies to V antigens.
The use of defined PcrV derivatives possessing distinct phenotypes may lead to a better understanding of the functional aspects of T3 needle-tip proteins and the development of therapeutic agents or vaccines targeting T3SS-mediated intoxication.
To avoid that these high levels of HlyA would mask any T6SS-mediated effects due to premature intoxication of the amoebae, we engineered T6SS-proficient or T6SS-deficient mutants in an hlyA-minus background.
Productive intoxication of a cell by ricin involves receptor-mediated uptake at the cell surface, retrograde routing via the Golgi complex to the endoplasmic reticulum (ER) lumen (5- 8), followed by retro-translocation of the catalytic subunit through Sec61 channels into the cytosol (9).
Its intractability is part of the intoxication of belief.
They probably also wouldn't appreciate the intoxication of a good beer..
The glittering intoxication of the champagne waxed philosophical.
The functional and regulatory information of this multifunctional translocator, PcrV, will facilitate the development of new-generation vaccines and therapeutics targeting on type III-mediated intoxication of host cells.
The proposed antiviral agents, which he denoted "sitoxins" (for "signal-regulated cleavage-mediated toxins"), were artificially composed of an intracellular signaling moiety that inactivates a toxin, e.g. a degradation signal, which can be cleaved off by a viral protease, resulting in a selective intoxication of virally infected cells [5].
The antibody-mediated delivery method illustrates the effectiveness in retargeting BoNT/B to human BMDMs; however, use of the intact toxin can potentially result in the intoxication of neuronal cells.
