Sentence examples for mediated integration of from inspiring English sources
We inserted the GFP-encoding sequence at the predicted 3′ end of the crb-3 gene, and generated transgenic lines carrying an integrated copy of this construct by gamma-irradiation mediated integration of an extrachromosomal array.
To determine further if φC31 integrase mediated integration of plasmid DNA into the cells that generated tumors, we tested all dissected tumors for integration into the dominant pseudo attP site in the mouse liver genome known as mpsL1 [9], using a nested PCR.
BC{−1} also proved to be capable of pseudo attB recombination, as it mediated integration of CbC P0 and P3 donor plasmids (pDCP and pDCbC-P3, respectively) into the genome (Fig. 6D).
Several physical factors such as chromosomal position, chromatin structure and transcriptional state of the target region affects the homologous recombination (HR) mediated integration of transforming DNA (Ninomiya et al., 2004; Feng et al., 2012).
The newly found mutated loxP pairs should be useful for Cre-mediated integration of transgenes and AGIS.
Concepts used in CHESS must be backed by a formal ontology in order to facilitate reasoner-mediated integration of chemical information.
In conclusion, we demonstrate that TALEN-mediated integration of a myelo-specific gp91phox transgene into AAVS1 of patient-derived iPSCs represents a safe and efficient way to generate autologous, functionally corrected granulocytes.
Transcriptional lacZY fusions were constructed by the pCP20-mediated integration of the pCE36 plasmid encoding a promoterless lacZY gene into unique FRT scars of selected genes [27].
gambiae targeting strains for phiC31 integrase were generated by piggyBac-mediated integration of the attP target site, linked to an eye-specific ECFP marker.
Several stable HeLa clones were generated by Flp recombinase-mediated integration of these doxycycline-responsive cassettes into a single pre-defined site in the Flp-In HeLa cells, a strategy that eliminates differences in expression levels that may arise because of integration at different chromosomal sites [36].
B) CRISPRm-mediated integration of the amplicons.
